Does a PCIe 4.0 Motherboard Impact Performance with a PCIe 5.0 GPU?

In short, if you run out of VRAM, then yes, the PCIe 4.0 limitations could kick in. Otherwise, you shouldn't notice much of a difference when gaming.

The PCIe platform is backwards compatible, so using a PCIe 5.0 GPU on a PCIe 4.0 motherboard is totally doable. However, it will be limited to the speeds of PCIe 4.0. And just a heads up, you might have mixed up 'PCLe'—it should actually be PCIe, which stands for Peripheral Component Interconnect Express.

Not really, at least not until you run out of VRAM. If you're playing demanding games that require more than 8 GB of VRAM, then your RTX 5050 could struggle since it operates on an x8 lane configuration. This could lead to performance issues compared to GPUs with full x16 lanes, like the RX 9060 XT 8 GB. Honestly, if you haven't bought the card yet, I'd suggest considering the RX 9060 XT instead; it typically offers better performance for a similar price.

Theoretical bandwidth will be halved when using PCIe 4.0 compared to PCIe 5.0, but this usually doesn't translate to significant gaming performance issues. Most likely, you won't even be able to tell the difference in actual gameplay.