How can I run Complete Anatomy on Debian Linux?

You could try running the Windows installation inside a Windows container such as WinBoat. That would let you use the native application without having a full Windows desktop open all the time, although it still depends on the program working correctly in that environment.

Check whether the service’s web app provides the features you need. It may work well in a browser, especially if hardware acceleration is enabled.

Microsoft Store applications often don’t behave like traditional Windows executables, so Wine, Proton, and Lutris may not be able to run this version properly. If the developer offers a standalone installer, an older release, or an Android version with the same features, those may be better options. Since your GPU is AMD, you could also investigate Waydroid or another Android runtime.

A Windows virtual machine is probably the most dependable fallback. You can try Bottles first, or investigate the Lutris log because error 256 is generic and doesn’t identify the actual failure. If those don’t work, a KVM virtual machine managed with virt-manager should let you install the application normally. Hardware-accelerated graphics may take extra setup, but your CPU and memory should be adequate for basic use.