Is It Okay to Leave One Motherboard Hole Without a Standoff?

No big deal if one hole is missing a standoff. Many new cases are designed with that in mind. Some boards have a hole for compatibility with older cases that had more standoffs, but if you don't have one there, just screw into the ones you do have without worry.

You're totally fine leaving that hole empty; having 9 screws will securely hold your motherboard in place. It's more about the stability from the available standoffs than hitting every single hole.

If you’ve got 9 standoffs, you should be good! Most motherboards only need a handful to stay secure, so 90% coverage is usually enough. Just make sure you have standoffs where the power connectors are for extra support.

Double-check your case package; there might be spare standoffs included. If you’re missing one, it won’t be a big issue as long as the critical areas have support, especially around where the power supply connects.