Does Disabling an Account Affect Out of Office Replies in Microsoft 365?

Nope, disabling the account doesn't affect the Out of Office replies. The mailbox continues to function even if the associated Active Directory account is disabled.

Simple test: create a shared mailbox, set an Out of Office reply, and then disable the account linked to it. The Out of Office will still work just fine. We typically disable the account, convert it to a shared mailbox, and set up the Out of Office message without any issues.

Short answer: Nah. Disabling an account just prevents login access, it doesn't stop the services. The Out of Office feature remains active since the mailbox isn't disabled. You can set it up in the mail settings within Microsoft 365 Admin.

It's pretty standard for us to keep users disabled for a period and still have their Out of Office replies active. Disabling the account only blocks the login, while the mailbox remains functional.

Definitely no! You can even have Out of Office replies set up on shared mailboxes, which don’t need an activated user account. So, nothing changes for Out of Office when you disable an account.